| Player | Pos | No. | Years |
|---|---|---|---|
| Derrik Allen | DB | 7 | — |
| Jayden Bellamy | DB | 29 | — |
| Noah Boykin | DB | 27 | — |
| D.M. Butler | DB | 7 | 2014 |
| Ramon Henderson | DB | 11 | 2021–2023 |
| Clarence Lewis | DB | 3 | 2020–2023 |
| Nick McCloud | DB | 4 | 2020 |
| Jaden Mickey | DB | 20 | 2022–2024 |
| Caleb Offord | DB | 2 | — |
| Munir Prince | DB | 7 | 2006–2007 |
| Isaiah Rutherford | DB | 2 | — |
| K.J. Wallace | DB | 7 | 2019–2021 |
| Ashton White | DB | 2 | 2016 |
| Temitope Agoro | CB | 26 | 2019 |
| Mike Anello | CB | 37 | 2008 |
| Josh Atkinson | CB | 24 | — |
| E.J. Banks | CB | 27 | — |
| Ryan Barnes | CB | 14 | — |
| Robert Blanton | CB | 12 | 2008–2011 |
| TaRiq Bracy | CB | 28 | 2018–2022 |
| Jalen Brown | CB | 21 | — |
| Devin Butler | CB | 12 | — |
| Shaun Crawford | CB | 20 | 2016–2020 |
| Isaiah Dunn | CB | 32 | 2025 |
| Matthias Farley | CB | 41 | 2012–2015 |
| Dallas Golden | CB | 14 | 2025 |
| Christian Gray | CB | 6 | 2023–2025 |
| Gary Gray | CB | 4 | 2008–2011 |
| Cam Hart | CB | 5 | 2020–2023 |
| Karson Hobbs | CB | 21 | 2024–2025 |
| Max Hurleman | CB | 37 | 2024 |
| Bennett Jackson | CB | 2 | 2010–2013 |
| Rashad Kinlaw | CB | 26 | — |
| Julian Love | CB | 27 | 2016–2018 |
| Cole Luke | CB | 36 | 2014–2016 |
| Raeshon McNeil | CB | 8 | 2008 |
| Leonard Moore | CB | 15 | 2024–2025 |
| Benjamin Morrison | CB | 20 | 2022–2024 |
| Troy Pride Jr. | CB | 5 | 2016–2019 |
| Cody Riggs | CB | 31 | 2014 |
| Philip Riley | CB | 25 | — |
| KeiVarae Russell | CB | 6 | 2012–2015 |
| Jamoris Slaughter | CB | 26 | 2010–2011 |
| DeVonta Smith | CB | 0 | 2025 |
| Lorenzo Styles Jr. | CB | 3 | 2021–2022 |
| Cree Thomas | CB | 20 | 2025 |
| Marcus Thorne | CB | 43 | 2020 |
| Chance Tucker | CB | 18 | 2023 |
| Donte Vaughn | CB | 8 | 2016–2019 |
| Darrin Walls | CB | 2 | 2007–2010 |
| Nick Watkins | CB | 9 | 2017 |
| Lo Wood | CB | 23 | — |
| Mark Zackery IV | CB | 24 | 2025 |
| Litchfield Ajavon | S | — | 2021 |
| Chris Badger | S | 16 | — |
| Nicky Baratti | S | 29 | 2012 |
| Taebron Bennie-Powell | S | 31 | 2025 |
| Jadon Blair | S | 11 | 2025 |
| DJ Brown | S | 2 | 2019–2023 |
| Sergio Brown | S | 31 | — |
| Antonio Carter | S | 8 | 2023 |
| Jordan Clark | S | 1 | 2024 |
| Nick Coleman | S | 24 | 2016–2018 |
| Austin Collinsworth | S | 28 | 2010–2013 |
| Jalen Elliott | S | 21 | 2016–2019 |
| Justin Ferguson | S | 15 | 2012 |
| Nicco Fertitta | S | 28 | 2016–2018 |
| Alohi Gilman | S | 11 | 2018–2019 |
| Leonard Gordon | S | 24 | — |
| Houston Griffith | S | 3 | 2018–2022 |
| Kyle Hamilton | S | 14 | 2019–2021 |
| Eilar Hardy | S | 4 | — |
| Thomas Harper | S | 13 | 2023 |
| Rod Heard II | S | 2 | 2024 |
| Ray Herring | S | 6 | 2009 |
| C.J. Holmes | S | 29 | 2017 |
| Tae Johnson | S | 9 | 2024–2025 |
| Brandon Joseph | S | 16 | 2022 |
| Brandon Logan | S | 29 | — |
| Ethan Long | S | 22 | 2025 |
| Dan McCarthy | S | 15 | — |
| Kyle McCarthy | S | 28 | 2007–2009 |
| Ben Minich | S | 13 | 2023–2025 |
| Zeke Motta | S | 17 | 2010–2011 |
| Patrick Pelini | S | 32 | 2019 |
| Spencer Perry | S | 27 | 2016 |
| Drew Recker | S | 39 | 2016 |
| Max Redfield | S | 10 | 2014–2015 |
| Derek Roback | S | 49 | — |
| Avery Sebastian | S | 4 | 2016 |
| Adon Shuler | S | 8 | 2023–2025 |
| Elijah Shumate | S | 22 | 2014–2015 |
| Harrison Smith | S | 22 | 2010 |
| Jalen Stroman | S | 7 | 2025 |
| Devin Studstill | S | 8 | 2016–2018 |
| Luke Talich | S | 28 | 2023–2025 |
| John Turner | S | 31 | — |
| Kennedy Urlacher | S | 28 | 2024 |
| Skip Velotta | S | 34 | 2021–2023 |
| Justin Walters | S | 22 | 2021–2022 |
| Xavier Watts | S | — | 2021–2024 |
| Mykelti Williams | S | 8 | — |
